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Heterodyne Fourier domain optical coherence tomography for full range probing with high axial resolution
Angular dispersion compensation for acoustooptic devices used for ultrashort-pulsed laser micromachining
Acousto-optic lens with very fast focus scanning
Multicolor Single-Molecule Spectroscopy with Alternating Laser Excitation for the Investigation of Interactions and Dynamics
Frequency-domain wide-field laser Doppler in vivo imaging
ULTRAFAST random-access scanning in two-photon microscopy using acousto-optic deflectors
Q-Switches (french article)
Basic building units and properties of a fluorescence single plane illumination microscope
Development of an atom gravimeter and status of the 10-meter atom interferometer for precision gravity measurement
Fast two-photon in vivo imaging with three-dimensional random-access scanning in large tissue volumes
Interference and crosstalk in double optical tweezers using a single laser source
Ultrafast random-access scanning in two-photon microscopy using acousto-optic deflectors
Frequency-domain wide-field laser Doppler in vivo imaging
Quantum Dot Photon Statistics
High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision
Millijoule pulse energy Q-switched short-length fiber laser
Double-pass acousto-optic modulator system
Optical phase Shifting in AOM
Multicolor Single-Molecule Spectroscopy with Alternating Laser Excitation for the Investigation of Interactions and Dynamics
Optical recording of electrical activity in intact
Pulse Shortening and scaling of passively mode-locked feedback-stabilised Nd:KWG laser
Video-rate computational heterodyne holography
Holographic imaging of surface acoustic waves
Non-destructive testing of composite plates by holographic vibrometry
Holographic laser Doppler imaging of microvascular blood
Holographic laser Doppler imaging of pulsatile blood flow
Singular value demodulation of phase-shifted holograms
Coherent beam combining of seven fiber chirped-pulse amplifiers using an interferometric phase measurement
Laser frequency shift up to 5 GHz with a high-efficiency 12-pass 350-MHz acoustooptic modulator
Fast optical recording of neuronal activity by three-dimensional custom-access serial holography
Abstract
Optical recording of neuronal activity in three-dimensional (3D) brain circuits at cellular and millisecond resolution in vivo is essential for probing information flow in the brain. While random-access multiphoton microscopy permits fast optical access to neuronal targets in three dimensions, the method is challenged by motion artifacts when recording from behaving animals. Therefore, we developed three-dimensional custom-access serial holography (3D-CASH). Built on a fast acousto-optic light modulator, 3D-CASH performs serial sampling at 40 kHz from neurons at freely selectable 3D locations. Motion artifacts are eliminated by targeting each neuron with a size-optimized pattern of excitation light covering the cell body and its anticipated displacement field. Spike rates inferred from GCaMP6f recordings in visual cortex of awake mice tracked the phase of a moving bar stimulus with higher spike correlation between intra compared to interlaminar neuron pairs. 3D-CASH offers access to the millisecond correlation structure of in vivo neuronal activity in 3D microcircuits.